Fig. 4

USP3 can influence the expression of SUZ12 protein in GC cells. a Overexpression of USP3 or the knock down of USP3 using three independent siRNAs did not affect the SUZ12 mRNA level in GC cells. *, P > 0.05. b Increasing amounts of USP3 were transfected into GC cells and SUZ12 expression was detected by Western blot. ***, P < 0.01; ****, P < 0.001. c The knock down of USP3 was achieved by transfecting cells with three independent siRNAs for 48 h. The expression of USP3 and SUZ12 was detected by Western blot. d Double staining for USP3 and SUZ12 in GC cells, as visualized by confocal microscopy, with the nuclei counterstained with Hoechst 33258. Scale bars represent 20 μm