Fig. 6

SUZ12 is involved in USP3-induced GC cell migration and invasion. a SUZ12 expression levels were detected using Western blot analysis in GC cells, which were transfected with USP3 overexpression plasmids. This was followed by transfection with SUZ12 siRNA1 or Scr siRNA. b Confluent AGS monolayers transfected with USP3 SUZ12 siRNA1 or USP3 scr siRNA were scratch- wounded at 72 h after transfection. Photographs were obtained 72 h after wound generation. ****, P < 0.001, USP3 scr siRNA vs. USP3 SUZ12 siRNA1. c Cell invasion of USP3 SUZ12 siRNA1 or USP3 Scr siRNA in GC cells was assessed by a Matrigel invasion chamber. The number of invaded cells in GC cells was counted. All values shown are mean ± SD of triplicate measurements and were repeated 3 times with similar results. ****, P < 0.001, USP3 Scr siRNA vs. USP3 SUZ12 siRNA1. d Morphology in USP3 SUZ12 siRNA1 or USP3 Scr siRNA cells as visualized by phase-contrast microscopy. e Representative IHC images are shown for USP3 and SUZ12 expression in lymph node metastatic cancer tissues. f Knock-down of SUZ12 in USP3-overexpressing cells inhibited the AKT/ERK/EMT signaling pathway as detected using Western blot analysis. Scale bars, 50 μm in d and 200 μm in e