Fig. 1
From: Functional and mechanistic studies reveal MAGEA3 as a pro-survival factor in pancreatic cancer cells
Under normal culture condition, ectopic expression of MAGEA3 has no effect on the pancreatic cancer cells’ proliferation but knockdown affects the viability in vitro. a-d MTT assay showing the rate of proliferation of the indicated pancreatic cancer cells, Y-axis showing the absorbance relative to Day 1 (D1); X-axis showing the indicated days. e Representative bright field micrograph showing change in morphology upon MAGEA3 depletion in BxPC3 cells, scale bar = 100 μm. f-i MTT assay showing the decrease in viable cells upon MAGEA3 knockdown (f), crystal violet stained plate image and the corresponding bar graph showing reduction in cell number upon MAGEA3 depletion (g; mean ± SEM, n = 3). AnnexinV and propidium iodide (FACS analysis) assay showing increased number of apoptotic and pre apoptotic cells upon MAGEA3 depletion (h and i). ** = p < 0.005, *** = p < 0.001, n = 3