Skip to main content
Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Functional and mechanistic studies reveal MAGEA3 as a pro-survival factor in pancreatic cancer cells

Fig. 2

MAGEA3 provides survival advantage to growth factor deprived pancreatic cancer cells in 2D cell culture model. a Bright field images of serum starved pancreatic cancer cells showing more number of cells when MAGEA3 is expressed ectopically. “Dox+” refers to doxycycline treatment (100 ng/mL). Scale bar = 200 μm. b MTT assay showing the fraction of viable cells at indicated condition. “Dox+” refers to doxycycline treatment (100 ng/mL). *** = p < 0.001, n = 3. c Representative crystal violet stained images for the indicated condition for 72 h. Dox refers to doxycycline treatment (100 ng/mL). The values represents Mean ± SEM relative to 10% FBS + Dox, n = 3. d Immunoblot showing level of apoptosis in ectopically expressing MAGEA3 and control cells in response to growth factor deprivation. e, f Representative crystal violet stained plate image along with the bar graph (e; Mean ± SEM, n = 3) and bright field image (scale bar = 100 μm) (f) for the indicated condition.g-i FACS analysis for AnnexinV and propidium iodide stained cells (g and h) and MTT assay (i) showing more cell death upon MAGEA3 depletion, * = p < 0.05, ** = p < 0.005, *** = p < 0.001, n = 3. j Immunoblot showing level of apoptosis in MAGEA3 depleted BxPC3 cells

Back to article page