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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Functional and mechanistic studies reveal MAGEA3 as a pro-survival factor in pancreatic cancer cells

Fig. 4

MAGEA3 is involved in cell survival pathway that helps the cancer cells to survive during growth factor deprivation. a, b Bright field micrographs (a, scale bar 200 μm, 10X objective) of AsPC1-GFP i.e. control cells and crystal violet stained plate images (b, Mean ± SEM, n = 3) showing the survival advantage provided by the conditioned media from AsPC1-MAGEA3-HA cells (72 h, fresh conditioned media after 24 h). CM, Conditioned media. The bar graph represents the quantification of the crystal violet assay. The percentage is calculated relative to the 10% FBS + doxycycline condition (The value is Mean ± SEM, n = 3). c, d Immunoblot showing up-regualtion of CCL2 and survivin upon ectopic expression of MAGEA3. Star above the band indicates the leaky expression of MAGEA3 or MAGEA3-HA protein. e The bar graph represents the concentration of human CCL2 present in the conditioned media of control or MAGEA3-HA overexpressing cells. D+ represents doxycycline treatment (100 ng/mL). *** = p < 0.001, n = 3. f-h Immunoblot (f and g) and qPCR (h) analysis confirmed the down regulation of survivin and CCL2 in BxPC3 cells upon MAGEA3 knockdown. i Immunoblot shows increased expression of survivin upon MAGEA3 expression in mouse pancreatic epithelial cells

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