Fig. 5

CCL2 neutralization reduced the cell viability in MAGEA3 non-expressing or MAGEA3-depleted cells. a Bright field images (scale bar = 100 μm, 20X objective) of pancreatic cancer cells shows decreased number of cells upon treatment with of CCL2 neutralizing antibody (2 ng/mL, every 24 h, 72 h treatment) in the absence of growth factors (0% FBS). b, c Crystal violet stained plate images showing the decreased number of cells upon CCL2 neutralization (anti-CCL2 antibody or IgG, 2 ng/mL, every 24 h, 72 h treatment) in both control and MAGEA3-expressing cells. In bar graph, the values represents Mean ± SEM,* = p < 0.05, n = 3. d, e Bright field images (d, scale bar 100 μm, 20X objective) and MTT assay (e) collectively shows the decrease in cell viability in response to CCL2 neutralization (anti-CCL2 antibody or IgG, 2 ng/mL, every 24 h, 72 h treatment) alone and further decreased upon MAGEA3 knockdown which can be reversed by supplementing with recombinant CCL2 (rCCL2, 1 ng/mL)