Fig. 6

GINS4 knockdown inhibits lung cancer migration and invasion in vitro. a-c A xenograft nude mouse model was established, and volumes of tumors derived from GINS4-knockout H1299 cells or control cells were determined at indicated time points (a). Images showing the size (b) and weight (c) of tumors isolated from nude mice injected with GINS4-depleted PC9 cells or control cells. d Hematoxylin and Eosin staining and immunohistochemistry staining for the indicated proteins of a representative primary xenograft originating from GINS4-depleted H1299 cells or control cells. Original magnification 400 ×, scale bar, 50 μm. e The MTT assay was performed to assess the viability of LSH-overexpressing and GINS4-depleted H1299 cells. f A representative image showing the migration (upper region) and invasion (lower region) of LSH-overexpressing and GINS4-depleted H1299 cells (n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001