Fig. 4

UBL4A causes lysosomal dysfunction in PDAC cells. a, b The immunofluorescence staining exhibited the collocation and expression of LC3B and LAMP1 in the cytoplasm in UBL4A overexpressed and downregulated groups (original magnification, 20×) (bars, 25 μm). c-f Lyso-Tracker Red DND-99 staining was performed on 4 PDAC cell lines of different groups (original magnification, 20×) (bars, 50 μm) and relative fluorescence intensity per cell was calculated. g-h Western blotting analyses of CTSB and UBL4A in four PDAC cell lines of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A) and relative band density were calculated. The statistical significance between different groups was calculated with Student t-test. Data are shown as the mean ± SD of three replicates; *P < 0.05, **P < 0.01; ***P < 0.001; ns: not significant