Fig. 5

UBL4A directly targets LAMP1 in PDAC. a Intersections among four online protein interaction databases. b Western blotting analyses of LAMP1 and UBL4A in four PDAC cell lines of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A). c-d The immunofluorescence staining exhibited the collocation and expression of UBL4A and LAMP1 in the cytoplasm in UBL4A overexpressed and downregulated groups (original magnification, 20×) (bars, 25 μm). e UBL4A was immunoprecipitated from cell lysates of four PDAC cells and LAMP1 was detected using LAMP1 antibody. f LAMP1 was immunoprecipitated from cell lysates of four PDAC cells and UBL4A was detected using UBL4A antibody. The statistical significance between different groups was calculated with Student t-test. Data are shown as the mean ± SD of three replicates; *P < 0.05, **P < 0.01; ***P < 0.001; ns: not significant