Fig. 2
From: A novel method to establish glucocorticoid resistant acute lymphoblastic leukemia cell lines
Morphological and biological characteristics of CEM-C7/HDR cell line. a Morphology of CEM-C7/HDR, CEM-C7/HDR-C2 and its parental CEM-C7–14 cells under phase contrast microscopy (× 400 magnification, scale bar 20 μM). b Wright-Giemsa staining of cells (× 1000 magnification, scale bar 20 μM). c Cell cycle distribution of CEM-C7/HDR, CEM-C7/HDR-C2 and CEM-C7–14 cells. Cells were cultured in a 6-well culture plate at 1 × 105/ml, and cell cycle progression was analyzed by PI staining after 48 h. Experiments were performed in triplicate. The percentage of cells in the G0/G1 and S phase showed no significant difference (P > 0.05) compared with each other. d Growth curves of CEM-C7/HDR, CEM-C7/HDR-C2 and CEM-C7–14 cells. Cells were cultured in a 6-well culture plate at 1 × 105/ml in RPMI-1640 medium with 10% FBS and grown for 7 days. Viable cells were counted using trypan blue staining every day. Experiments were performed in triplicate