Fig. 4
From: A novel method to establish glucocorticoid resistant acute lymphoblastic leukemia cell lines
Expression of GC-resistance- and hypoxia-associated proteins in CEM-C7/HDR. a Cells were lysed, and extracts were analyzed by western blotting for GR, p-GR (Ser211), AMPK and p-AMPK (Thr172). β-Actin was used as an internal control. Bar graphs show the ratio of proteins to β-Actin. For all experiments, values of triplicate experiments are shown as the mean ± SD. *: p < 0.01 versus CEM-C1–15, CEM-C7–14 and CEM-C7/H. #: p < 0.01 versus CEM-C7–14 and CEM-C7/H. †: p < 0.01 versus CEM-C7–14 and CEM-C7/H. +: p < 0.01 versus CEM-C1–15 and CEM-C7–14. b Western blot analysis of Glut-1, HKII, LDH and p-LDH (Tyr10). β-Actin was used as an internal control. Bar graphs show the ratio of protein to β-Actin. *: p < 0.01 versus CEM-C1–15, CEM-C7–14 and CEM-C7/H. #: p < 0.01 versus CEM-C7–14. c Western blot analysis of 4E-BP1, p-4E-BP1 (Thr37/46), p70S6K and p-p70S6K (Thr389). β-Actin was used as an internal control. Bar graphs show the ratio of protein to β-Actin. *: p < 0.01 versus CEM-C1–15, CEM-C7–14 and CEM-C7/H