Fig. 5
From: A novel method to establish glucocorticoid resistant acute lymphoblastic leukemia cell lines
Tumorigenicity of CEM-C7/HDR cells. a HE staining of the tumors derived from CEM-C7–14 and CEM-C7/HDR showed that the xenografts were composed of leukemia cells and blood vessels. Original magnification: × 400 magnification. b-e On IHC staining, both tumors derived from CEM-C7–14 and CEM-C7/HDR expressed GR, Glut-1, HKII, and 4E-BP1 proteins with a similar pattern, respectively. Original magnification: × 400 magnification. f CEM-C7/HDR or CEM-C7–14 cells were subcutaneously inoculated into BALB/C nude female mice. Once palpable tumors were established, animals were treated with 15 mg/kg/d Dex (Dex group), or PBS (Control group) for 14 days. Tumor volume was measured every 2 days, and data are expressed as mean volume ± SD (n = 4 tumors per group). Dex treatment had little effect on CEM-C7/HDR xenografts in nude mice, whereas inhibited tumor growth significantly in CEM-C7–14 xenografts mice. *: p < 0.05 versus CEM-C7–14 PBS group, CEM-C7/HDR PBS and Dex group