Fig. 2From: NKAP alters tumor immune microenvironment and promotes glioma growth via Notch1 signalingEffects of NKAP on the migration, invasion and EMT of glioma cells. a, Cell migration and invasion were determined by a transwell assay. Quantitative analysis showed that NKAP knockdown reduced glioma cell migration and invasion in different cell lines. ***P < 0.005 vs. Con. Levels of the representative EMT markers including N-cadherin, Twist1 and Vimentin were measured by qRT-PCR (b) and western blot (c) respectively. GAPDH was used as an internal control. Scale bar = 20 μm. The results represent the mean ± s.d. of three independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05Back to article page