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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: NKAP alters tumor immune microenvironment and promotes glioma growth via Notch1 signaling

Fig. 4

NKAP altered recruitment and polarization of TAMs via regulating the secretion of SDF-1 and M-CSF. a, Representative micrographs of mouse brain sections 35 days after intracranial implantation of U87 cells transfected with NKAP-siRNA or control vectors. Expression of SDF-1 was inhibited in NKAP knockdown glioma tissues. Levels of SDF-1 were measured by qRT-PCR (b) and western blot (c) in the cells transfected with NKAP-siRNA or control vectors. FACS quantification (d) and representative bar plots (e) demonstrated a decrease in the proportion of TAMs (i.e. CD206high, CD80low) following a co-incubation with NKAP knockdown U87 and U251 cells. f, Knockdown NKAP reduced the secretion of M-CSF in the glioma cells. g and h, Analysis of TCGA database about glioma tissues (n = 370) shows significant correlation between expressions of NKAP and M-CSF (CSF1)/SDF-1 (CXCL12). Scale bar = 20 μm. Data are presented as the mean ± s.d. of three independent experiments. ***P < 0.001

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