Fig. 7

Blockage of Notch1 weakened the effects of NKAP on the proliferation and invasion of glioma cells. The U87 and U251 cell lines were transfected with NKAP plasmids to overexpress NKAP, which were termed NKAP OE cells. DAPT was also applied to the transfected cell lines. a, The growth curves of the transfected cells plotted based on a CCK8 assay. After inhibiting Notch1 signaling by DAPT, the cell viability was decreased compared with that of NKAP OE cells. Data are presented as the mean ± s.d. of three independent experiments. b, Cell proliferation was determined by an EdU staining assay. c, Cell invasion was determined by a transwell assay. The results represent the mean ± s.d. of three independent experiments. Scale bar = 20 μm. The results represent the mean ± s.d. of three independent experiments. ***P < 0.001