Fig. 1

BEZ235 suppresses growth of NSCLC cells. a BEZ235 blocks proliferation of NSCLC cell lines expressing either wild-type EGFR or activating mutants of EGFR. Proliferation of NSCLC cells treated with vehicle control (ctrl) or 100 nM BEZ235 (BEZ) was assessed using the WST-1 assay. A549, CL141, CL83, ZSY, LIJ and CL152 cell lines expressed wild-type EGFR, whereas PC9, PC9-IR, CL25, H3255, H1975 and CL97 cell lines expressed activating mutants of EGFR. Of these, PC9-IR, H1975 and CL97 were resistant to gefitinib, and CL25 was partially sensitive to it. Three independent experiments were performed in triplicate. Values are reported as means ± SD (*, P < 0.05; **, P < 0.01; ***, P < 0.001; Student’s t-test). b BEZ235 suppresses the anchorage-independent growth of both EGFR-wild type and EGFR-mutant NSCLC cells. Cells were seeded at 500 cells/plate and grown in soft agar in medium containing vehicle (DMSO) or 100 nM BEZ235 for 14 days, after which colonies were photographed and counted. Three independent experiments were performed in triplicate. Values are reported as means ± SD (*, P < 0.05; **, P < 0.01; ***, P < 0.001; Student’s t-test)