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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: MicroRNA-486-3p functions as a tumor suppressor in oral cancer by targeting DDR1

Fig. 4

The effects of miR-486-3p overexpression on OSCC cells a: Western blot analysis of DDR1, Bcl-2, cleaved caspase 3 and PARP in OEC-M1 and TW2.6 cells following miR-486-3p (PM-486-3p) overexpression for 48 h. GAPDH was used as an internal control. b: Colony formation assay after miR-486-3p (PM-486-3p) transfection in OEC-M1 and TW2.6 cells for 10 days (left). The mean number of colonies for each well was determined from three independent assays (right). c: Growth rates of OEC-M1 and TW2.6 cells measured by MTT assay after miR-486-3p (PM-486-3p) transfection. d: Left: Dot plot for flow cytometric analysis of apoptotic cells after miR-486-3p (PM-486-3p) transfection in OEC-M1 and TW2.6 cells for 48 h. Annexin V/PI: viable cells (lower left quadrant), Annexin V/PI+: necroticcells (upper left quadrant), Annexin V+/PI: early apoptotic cells (lower rightquadrant), Annexin V+/PI+: late apoptotic cells (upper right quadrant). Right: Bar graph quantifying the percentage of, early apoptotic (early), late apoptotic (late) and necrotic cells (necrosis) according to miR-486-3p (PM-486-3p) transfection. All data are presented as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001 versus negative control (NC)

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