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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MicroRNA-486-3p functions as a tumor suppressor in oral cancer by targeting DDR1

Fig. 5

Methylation status of ANK1 promoter in oral cancer a: Methylation specific PCR of ANK1 promoter region in OEC-M1 and TW2.6 cells. Primers arespecific for unmethylated (U) or methylated (M) DNA.b: OEC-M1 and TW2.6 cells were treated without or with 5-Aza-dC (5 μM) for 5 days. Methylation status of ANK1 promoter region were measured by methylation specific PCR. c: qRT-PCR analysis of ANK1 expression after 5-aza-dC treatment in OEC-M1 and TW2.6 cells (mean ± SD; ***p < 0.001). d: qRT-PCR analysis of miR-486-3p expression after5-aza-dC treatment in OEC-M1 and TW2.6 cells (mean ± SD; *p < 0.05; ***p < 0.001). e: Western blot analysis of DDR1 protein after 5-Aza-dC (5 μM) treatment for 5 days. GAPDH was used as an internal control. f: Growth curves of OEC-M1 and TW2.6 cells measured by MTT assay after 5-Aza-dC (5 μM) for indicated time (mean ± SD; ***p < 0.001). g: Left: Colony formation assay after 5-Aza-dC (5 μM) treatment in OEC-M1 and TW2.6 cells for 10 days. Right: The mean number of colonies for each well was determined from three independent assays. All data are presented as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001 versus control (NC)

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