Fig. 4

TXNDC5 is required for HERG1-mediated proliferation, invasion, and EMT in ESCC cells. (a and b) TE-1 cells transfected with a HERG1-overexpressing or control plasmid, and KYSE-30 cells transfected with HERG1 or scramble shRNA were subjected to qPCR (a) and western blot analyses (b) for the detection of HERG1 and TXNDC5 expression (n = 3). (c and d) MTT assays and Boyden chamber invasion transwell assays were used to assess cell proliferation (c) and invasion (d) in TE-1 cells overexpressing HERG1 and/or silenced for TXNDC5, and KYSE-30 cells silenced for HERG1 and/or overexpressing TXNDC5 (n = 3). (e) TE-1 and KYSE-30 cells in different treatment groups were subjected to qPCR for the detection of HERG1, TXNDC5, p21, cyclin D1, E-cadherin, vimentin, and fibronectin expression (n = 3). *: p < 0.05, #: p < 0.05 vs. HERG1 or HERG1 shRNA