Fig. 6

PI3K/AKT signaling pathway plays an important role in HERG1-dependent TXNDC5 upregulation. a HERG1-overexpressing TE-1 cells and HERG1-knockdown KYSE-30 cells were analyzed by western blotting to assess the expression of HERG1 and accumulation of JNK1/2, p38, PI3K, AKT, and Src, as well as their phosphorylated forms (n = 3). (b and c) HERG1, TXNDC5, p21, cyclin D1, E-cadherin, vimentin, and fibronectin expression in TE-1 cells administered the PI3K/AKT inhibitor LY294002 for 24 h in the presence or absence of HERG1 overexpression according to qPCR (b) and western blot analyses (c). HERG1, TXNDC5, p21, cyclin D1, E-cadherin, vimentin, and fibronectin expression in KYSE-30 cells transfected with HERG1 shRNA and/or AKT cDNA according to qPCR (b) and western blot analyses (c) (n = 3)