Fig. 7

HERG1 boosts tumor growth and metastasis in athymic mice. a KYSE-30/scramble, KYSE-30/shHERG1 #1, or KYSE-30/shHERG1 #2 tumor cells were subcutaneously injected into the back of female athymic mice to evaluate tumorigenesis. Twenty-eight days post-implantation, the volumes of the generated tumors were compared among the three groups (n = 10). b Tumor volumes were measured in the three experimental groups over 28 days (n = 10). c Representative images from immunohistochemical analysis of tumor samples stained with H&E and with a HERG1 and Ki-67 antibody (n = 10). Magnification: 200×. d Western blotting relative to the expression of HERG1, TXNDC5, p21, cyclin D1, E-cadherin, vimentin, and fibronectin in the subcutaneous tumors (n = 3). e Representative bioluminescence imaging of nude mice and ex vivo imaging of the lungs and photographs and H&E staining of metastatic tumors in lung samples harvested on day 35 after caudal intravenous injection of ESCC cells from the three groups (n = 10). Magnification: 100×. *: p < 0.05