Fig. 2

Knockdown of ATG4C inhibited the proliferation of glioma cells. a-b ATG4C siRNAs interference efficiency was tested at both mRNA and protein levels. c ATG4C knockdown by si-ATG4C transfection suppressed the proliferation of glioma cells in a time-dependent manner. d ATG4C knockdown by si-ATG4C transfection impaired clone formation ability of glioma cells. Left panel: representative crystal violet staining images; right panel: quantitative analysis of colony numbers. *p < 0.05, **p < 0.01, ***p < 0.001, NS: not significant