Fig. 5

High glucose promotes proliferation and suppresses apoptosis by upregulating SREBP1. a-d After pretreatment with SiSREBP1 and culture with normal glucose and high glucose, the SREBP1 expression was detected by western blotting assays. Colony formation assays (e, f) and flow cytometry (g, h) were used to evaluate the effect of SiSREBP1 on colony-forming ability and apoptosis. (i-l) The expression of proliferation and apoptosis markers was detected by western blotting assays after pretreated with SiSREBP1 and culture under normal glucose and high glucose for 48 h. n = three independent experiments, *P < 0.05, **P < 0.01, or ***P < 0.001