Fig. 5

Napabucasin downregulates RelA and reduces constitutive p-RelA in GBM cells. a Bubble chart of upregulated genes analyzed by KEGG/GO. The top 20 enrichment pathways are displayed on the Y-axis. The bubble size represents the number of genes, and the bubble color represents the -log(p-value). b Correlations between STAT3 and RelA were analyzed in CGGA and TCGA databases. c Immunofluorescence (IF) localization of STAT3 (p-STAT3) and RelA (p-RelA) in U87MG and LN229 cells. Localization of STAT3 (p-STAT3) and RelA (p-RelA) is shown by red and green fluorescence, respectively. Colocalization of STAT3 (p-STAT3) and RelA (p-RelA) is shown by yellow fluorescence. Nuclei were stained with DAPI (blue). Scale bar = 50 μm. d IF of the expression and location of total RelA (t-RelA) and phospho-Ser536-RelA (p-RelA) in GBM cells after treatment with 5 μM Napabucasin for 48 h. Nuclei were stained with DAPI (blue). Scale bar = 50 μm. e U87MG and LN229 cells were treated with Napabucasin (5 μM) or DMSO (0.1%) for 48 h. Then, cell lysates were analyzed by western blotting using antibodies against t-RelA and p-RelA. (*p < 0.05, **p < 0.01, #p < 0.001)