Fig. 3

Effect of 6G on vascular normalization in zebrafish model and vascular remodeling in HUVECs. a Representative zebrafish retinal angiogenesis in normal, hypoxia and hypoxia/6G group. **, P < 0.01, one-way ANOVA. b Closed vessel, abnormal branches, sprouts and fractal dimension values in normal, hypoxia and hypoxia/6G group of zebrafish retinal vessels. *, P < 0.05; **, P < 0.01, one-way ANOVA. 6G exerted a protective effect against hypoxic damage. c Effect of 6G on tube formation in HUVECs. *, P < 0.05; one-way ANOVA. d Coefficient of variation of tube. 6G decreased the coefficient of variation among tubes. e Average area of tube. 6G increased tube area. f SEM and fluorescence staining showing the effect of 6G on HUVECs. g 6G promotes endothelial cytoskeleton remodeling and regulates pseudopod connections of HUVECs. *, P < 0.05; **, P < 0.01, one-way ANOVA. Results were obtained from three independent experiments, each performed in triplicate, and the error bars represent SD. Data are represented as the means ± SEM