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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

Fig. 3

Cytotoxic effect of RHPS4 in single treatment and in combination with IR. Images of U251MG-derived neurospheres treated with increasing concentrations of RHPS4 (0.2–1 μM for 5 days) and then exposed to 10 Gy of X-rays (a). Neurospheres maximal surface was automatically calculated by IS-Capture software after manual surrounding of each sphere. Maximal surface data were grouped in 5 different classes (0–10, 11–20, 21–30, 31–40 and ≥ 41*102 μm2) for both unirradiated and 10 Gy-exposed U251MH-Sph cells. Data have been reported as percentage of the total number of spheres analyzed and represent mean values ± s.d. (n = 3) (b). Spheres number and max surface in samples exposed to RHPS4 and IR were shown in (c) and (d), respectively. Data represent mean values ± s.d. (n = 3). Growth curves of GSCs from patients treated with RHPS4 (1, 2, 3 and 4 μM) and followed for 8 days (e). Data represent mean values ± s.d. (n = 2). Effect of RHPS4 and γ-rays combined treatment on cell growth in GSCs from patients. Cell growth was evaluated after 3 (f) and 7 days (g) from irradiation. Combined treatment was performed treating cells with IC25 calculated at 4 days and then exposing them to 10 Gy γ-rays. Data represent mean values ± s.d. (n = 2). * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t-test)

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