Fig. 4

RHPS4 induces telomeric fusions and telomeric fragility in glioblastoma differentiated cells but not in GSCs. Representative images of telomere fusions involved in the formation of dicentric, tricentric and ring chromosomes observed in the U251MG-Adh cell line treated with 0.5 μM RHPS4 for 5 days (a). Frequency of classical dicentrics (dic) and dicentrics generated from telomere fusions (tel fus) in the U251MG derived cell lines and in GSCs lines from patients (b). Data represent mean values ± s.d. (n = 2). Representative images of U251MG-Adh cells in which are present several fragile telomeres (surrounded by boxes). Some of them were enlarged on the right side of the Fig. (c). Frequency of fragile telomeres per chromosome in the U251MG derived cell lines and in GSCs lines obtained from patients treated or not with RHPS4. Data represent the frequency of each metaphase scored and black bars denotes mean values (d). (n = 2). * P < 0.05 (Student’s t-test)