Table 1 Combined RHSP4 and irradiation treatment protocols
Target (biological model) | RHPS4 administration | IR exposure |
|---|---|---|
Xenograft GBM tumors in mice | Intravenous injection in caudal vein of 10 mg/kg/die RHPS4 for 5 days or 10 days | At day 5 or 10 tumors were exposed to 10 Gy of X-rays (mice were shielded using appropriate lead plates in order to irradiate only the tumor mass) |
U251MG-Adh or U251MG-SC-Sph | RHPS4 0.2, 0.5 or 1 μM was added in the culture medium and maintained for 5 days | At day 5 cells were exposed to 10 Gy of X-rays (250 kV, 6 mA) with a dose rate of 0.53 Gy/min |
Patient-derived GSCs | RHPS4 was added in the culture medium and maintained for 4 or 8 days | At day 4 cells were exposed to 10 Gy of γ-rays (cesium-137 γ-irradiation) with a dose rate of 0.8 Gy/min |