Fig. 1
APE1 and NPM1 are involved in the response to CDDP cytotoxicity. a Representative western blotting on whole cell extracts (WCE) of CH12F3 cells shows the different amount of APE1 in CH12F3 APE1+/+/Δ and the isogenic knock out CH12F3 APE1Δ/Δ/Δ. On the right side of each panel, the Molecular Weights (MW), expressed in kDa, are indicated. On the left side of each panel, specific antibodies used in the immunoblotting are indicated. Actin was used to normalize APE1 levels. Histograms show the decreased viability of both cell lines treated with the indicated doses (μM) of CDDP for 24 h. Values express the mean viability ± SD from at least three independent replicates. Each value is normalized to the untreated condition. ***p < 0.001. b Representative western blotting on whole cell extracts (WCE) of MEF cells shows the different amount of NPM1 in MEF NPM1+/+ and the isogenic knock out MEF NPM1−/−. On the right side of each panel, the Molecular Weights (MW), expressed in kDa, are indicated. On the left side of each panel, specific antibodies used in the immunoblotting are indicated. Actin was used to normalize NPM1 levels. Histograms show the decreased viability of both cell lines treated with the indicated doses (μM) of CDDP for 24 h. Values express the mean viability ± SD from at least three independent replicates. Each value is normalized to the untreated condition. *p < 0.05, **p < 0.01, ***p < 0.001. c Representative western blotting on whole cell extracts (WCE) of OCI-AML cells shows the presence of NPM1c + in OCI-AML3 compared to the counterpart OCI-AML2. On the right side of each panel, the Molecular Weights (MW), expressed in kDa, are indicated. On the left side of each panel, specific antibodies used in the immunoblotting are indicated. Actin was used to normalize APE1 levels. Histograms show the decreased viability of both cell lines treated with the indicated doses (μM) of CDDP for 24 h. Values express the mean viability ± SD from at least three independent replicates. Each value is normalized to the untreated condition. ***p < 0.001