Fig. 4
Overexpression of LEF1 enhances the tumorigenicity of ESCC cells in vivo. a, b Representative tumors from mice injected with 106 LV-LEF1 or LV-GFP ECA109 cells after 6 weeks were presented. Tumor volumes from each group were measured weekly. c Mice were injected with the indicated number of LV-LEF1 or LV-GFP ECA109 cells and tumor incidence in the mouse xenografts were obtained after 28 days. d, e Western blot (d) and Immunohistochemistry (e) analysis the LEF1 and OV6 expression on mouse subcutaneous tumors inoculated after 6 weeks. Scale bar = 50 μm. f-j Indicated number of LV-shLEF1 and LV-GFP ECA109 cells were injected into mice. Tumor volumes (f, g), tumor incidence (h), expression level of LEF1 and OV6 (i, j) were presented. Scale bar = 50 μm