Fig. 2

CDK5 related to HCC cell proliferation, migration and invasion. a Expression of CDK5 in 8 HCC cell lines by immunoblotting analysis. b Immunoblotting analysis of expression of CDK5 protein in Huh7 cells after infection of GFP-N1 and GFP-CDK5 lentivirus. c Immunoblotting analysis of expression of CDK5 protein in 7721 cells after transfection of shcdk5 or empty vector. d Cell counting assay in CDK5 stably expressing Huh7 cells for 5 days, t-test ***p < 0.001, n = 3. e Cell counting assay in shCDK5 SMMC-7721 cells for 5 days. t-test ***p < 0.001, n = 3. f Colony formation assay in CDK5 stably expressing Huh7 cells and control cells, t-test ***p < 0.001, n = 3. g Colony formation assay in SMMC-7721 cells after infection of shCDK5 or empty vector, t-test ***p < 0.001, n = 3. h migration(24 h) and i invasion(48 h) assay in Huh7 cells transfected with GFP-CMV, GFP-CDK5,GFP-CDK5-KD constructs; One Way ANOVA on Ranks,*P < 0.05; **P < 0.01;***P < 0.001. j migration (24 h) and k invasion (48 h) assays in SMMC-7721 cells after infection of shCDK5 or empty vector. The number of migrated and invaded cells were counted and the quantifications were present on right panel. t test,*P < 0.05; **P < 0.01;***P < 0.001