Fig. 7
From: CDK5-mediated phosphorylation and stabilization of TPX2 promotes hepatocellular tumorigenesis
CDK5 promotes HCC development through TPX2. a Migration(24 h) assay in SMMC-7721 cells transfected with GFP, GFP-wild-type TPX2 and non- phosphorylatable TPX2 (S486A) constructs; One Way ANOVA on Ranks, **P < 0.01; ***P < 0.001. b Migration(24 h) assay in SMMC-7721 cells transfected with shcon, shcdk5 and shcdk5 + TPX2. One Way ANOVA on Ranks, *P < 0.05. c Migration(24 h) assay in Huh7 cells transfected with CMV + sicon, CMV + sitpx2, Cdk5 + sicon, Cdk5+ sitpx2, One Way ANOVA on Ranks,*P < 0.05; **P < 0.01;***P < 0.001. d Immunoblotting analysis of TPX2 protein in xenograft tumors of CDK5 overexpression stable HCC Huh7 cells and control cells. e Immunoblotting analysis of TPX2 protein in xenograft tumors of shcon and shcdk5 stable cells. f Immunoblotting analysis of TPX2 and CDK5 protein in Human HCC tissues specimens(T) and non-cancerous surrounding tissues(N). Correlation analysis of TPX2 and CDK5 protein in Human HCC tissues was presented in the right panel