Fig. 1

TGF-β participates in the regulation of CTSL mediated paclitaxel resistance in A549 cells. a Western blot determined the expression of TGF-β of three cell lines. b A549 cells were treated with 10 μM TGF-β and harvested at different times, western blot was performed to detect the expression of CTSL protein level. c Western blot detected the expression of TGF-β and CTSL of A549/TAX cells transfected with TGF-β siRNAs targeting the human TGF-β sequence or the control siRNA. d and e CCK8 and flow cytometry assays were conducted to measure the change of paclitaxel resistance of A549/TAX cells. f Western blot was conducted to measure the expression level of TGF-β/smads signaling pathway associated proteins of three cell lines. g A549 cells were treated with different concentration of paclitaxel and harvested at 12 h, 24 h and 48 h, and western blot detected the expression level of TGF-β/smads signaling pathway associated proteins. At least three independent experiments were performed. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with control