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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: ADAR1 promotes the epithelial-to-mesenchymal transition and stem-like cell phenotype of oral cancer by facilitating oncogenic microRNA maturation

Fig. 3

Effect of ADAR1 on EMT of OSCC cell lines. a Western blot analysis of CDH1 and VIM in the LV-ADAR1 and LV-GFP groups. b CDH1 and VIM protein expression was detected by immunofluorescence analysis. c Western blot analysis of CDH1 and VIM in both si-ADAR1-p110 and si-ADAR1-p150 groups. d Representative image of immunofluorescence indicating that ADAR1-p110 knockdown attenuated EMT in OSCC cell lines. e Western blot analysis of CDH1, VIM and ADAR1 in OSCC cell lines treated with TGF-β for 0, 12, and 24 h. f Western blot analysis of CDH1 and VIM in the Scramble and si-ADAR1-p110 groups with 24 h TGF-β treatment. g The nuclear (ADAR1-N) and cytoplasm (ADAR1-C) expression levels of ADAR1 were measured in OSCC cell lines treated with TGF-β for 0, 12, and 24 h by western blot analysis. h Representative image of immunofluorescence showing ADAR1 expression in the process of TGF-β treatment. Scale bars: 20 μm. The data from at least three independent experiments were expressed as the mean ± SEM. *p < 0.05; **p < 0.01; #p > 0.05

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