Fig. 5

ADAR1 could bind to Dicer and promote the maturation of oncogenic miRNAs. a Representative image of Co-IP. Cell lysates were incubated with normal rabbit IgG, anti-ADAR1, anti-Dicer, anti-Drosha, anti-Argonaute1, anti-Argonaute2, and anti-TRBP antibodies. b Representative image of immunofluorescence indicated that ADAR1 and Dicer were co-located in Cal27 cells. Scale bar: 20 μm. c ADAR1 overexpression upregulated oncogenic miRNAs. d ADAR1 overexpression decreased the precursor levels of six oncogenic miRNAs. e ADAR1 overexpression decreased the primary levels of six oncogenic miRNAs. f ADAR1-p110 knockdown reduced oncogenic miRNAs and tumor suppressor miRNAs expression. g Dicer knockdown inhibited oncogenic miRNAs and tumor suppressor miRNAs expression. The data were expressed as the mean ± SEM (n ≥ 3). *p < 0.05; **p < 0.01; ***p < 0.00; #p > 0.05