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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: DHRS2 mediates cell growth inhibition induced by Trichothecin in nasopharyngeal carcinoma

Fig. 4

DHRS2 alters lipid profiling to induce cell cycle arrest in NPC cells. a The cell distribution was determined by flow cytometry in HK1-DHRS2 and C666–1- shDHRS2 cells compared with each vector control cells. The protein levels of cyclinD1, CDK4 and pRB (Ser780) were detected by western blot in HK1-CON / HK1-DHRS2 (b) and C666–1-CON / C666–1-shDHRS2 (c) cells. β-actin was used as a loading control. d The relative cellular content of FAs in HK1-CON / HK1-DHRS2 cells was analyzed by GC-MS. e-f Cell proliferation rate of each designated group was analyzed by MTS assay. After 16 h of starvation, HK1-CON and HK1-DHRS2 cells were treated in the absence or presence of 62.5uM OA or 62.5uM EA for 72 h as designated in each group and applied for MTS assay. g The protein levels of cyclinD1, CDK4 and pRB (Ser780) were detected by western blot assay. HK1-CON and HK1-DHRS2 cells were treated as (e-f) and applied for western blot. Data are shown as mean values ± S.D. of independent, triplicate experiments. The asterisks (*,**,***) indicate significant differences (p < 0.05, p < 0.01, p < 0.001,respectively)

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