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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: DHRS2 mediates cell growth inhibition induced by Trichothecin in nasopharyngeal carcinoma

Fig. 6

DHRS2 mediates growth inhibition of NPC cells induced by TCN. a Heatmap of the transcriptome changes upon TCN treatment (TCN) in comparison with the DMSO control (CON) in HK1 cells. b Scatter plots of all expressed genes in TCN treated group (TCN) compared with the control (CON). Red color means up-regulated gene, blue means down-regulated gene, and gray means non-regulated gene. The ‘regulated gene’ is defined as that with FDR ≤0.001 and abs(log2(Y/X)) ≥ 1. The mRNA (c) and protein (d) levels of DHRS2 in HK1 and C666–1 cells with different doses of TCN treatment. e The cell distribution was determined by flow cytometry in HK1 and C666–1 cells with different doses of TCN treatment. The protein levels of cyclinD1, CDK4, RB and pRB (Ser780) were detected by western blot assay in HK1 (f) and C666–1 cells (g) with different doses of TCN treatment. β-actin was used as a loading control. h Cell proliferation rate of each designated group was analyzed by MTS assay in C666–1-CON or C666–1-shDHRS2 cells in the absence or presence of 0.5uM TCN. Cell proliferation rate of each designated group was analyzed in HK1 (i) and C666–1 (j) cells. Cells were treated in the absence or presence of 0.5uM TCN, 62.5uM OA or 62.5uM EA for 72 h as designated in each group and applied for MTS assay. Data are shown as mean values ± S.D. of independent, triplicate experiments. The asterisks (**,***) indicate significant differences (p < 0.01, p < 0.001,respectively). NS, no significance

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