Fig. 5
From: Flubendazole demonstrates valid antitumor effects by inhibiting STAT3 and activating autophagy
Flubendazole induces tumor cell apoptosis. (a) CRC cells were treated with the indicated concentrations of flubendazole and incubated for 48 h. Cells were stained with Annexin V and propidium iodide (PI), and then analyzed by flow cytometry. (b) CRC cells were incubated with flubendazole for 36–48 h, caspase 3 activity in the cell extracts was determined with a specific assay kit. (c) The apoptotic morphological characteristics of cells and cells stained with Hoechst staining were observed in RKO and HCT116 cells cultured with flubendazole (0, 0.3, 0.6 and 1.2 μM) for 12 h (200×). The images shown are representative of more than three separate experiments (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)