Fig. 1

p68 and RelA abundance is positively correlated and RelA protein expression is regulated by p68 in human colon cancer. a Representative images depicting immunohistochemical (IHC) staining of p68, RelA and PCNA conducted in tissue sections derived from human normal colon and colon carcinoma samples. Images were captured at 200X magnification. b Notched box plots showing the distribution of H-scores of p68 and RelA in normal (n = 22) and colon carcinoma (n = 45) samples; Mann–Whitney U-values were calculated from H-scores. c Comparison of the combined average H-scores of p68 and RelA. Error bars represent the mean (+) s.d.; P < 0.0001 is represented as ****; calculated using Student’s t-test. d Scatter plots representing the mean H-scores of p68 and RelA in normal and colon carcinoma tissue samples, respectively. e Determination of Spearman’s rank correlation coefficient (rs) between the mean H-scores of p68 and RelA, analyzed from both normal and colon carcinoma tissues in combination. f Multiple colon cancer cell lines as depicted in figure, grown in 60 mm cell culture dishes were transfected with 4 μg of pGZ-p68 or the empty vector (EV). g HCT 116 cells were seeded in 60 mm plates and were transfected with EV or pGZ-p68 in a dose-dependent manner as indicated. For both (f) and (g) cell lysates were prepared, 36 h post transfection and probed for the indicated proteins by immunoblotting (IB). ‘Exo’ and ‘Endo’ depicts exogenous and endogenous p68, respectively. Densitometry values below the blots in both panels (f) and (g) are relative to the loading control (Actin). h Multiple colon cancer cell lines were seeded in 60 mm cell culture dishes and transfected with 4 μg of either scramble shRNA (represented as scr), p68 shRNA1 or p68 shRNA2 (represented as sh p68–1 and sh p68–2 respectively) plasmids. Cell lysates were prepared, 48 h post transfection and probed for the indicated proteins by IB. Densitometry values relative to loading control (GAPDH/Actin) are given below the blots