Fig. 6

p68 overexpression in mice colorectal allograft model leads to enhanced primary tumor growth. a Mice syngeneic colon cancer cells-CT26 was transfected with either 4 μg of pEGFP-p68 or EV. G418 (500 μg/ml) was used for selection and maintenance of stable cells (CT26^EV and CT26^p68). Cell lysates were prepared, and probed for the indicated proteins by IB. Densitometry values relative to loading control (Actin) are given below the blots. b Representative images of the mice bearing the primary tumor generated after 30 days of inoculation of stable CT26^EV and CT26^p68 cells into the flank region of BALB/c (top panel). Arrows indicate the tumors. Corresponding H&E-stained images of the respective tumor sections captured at 200X magnification (bottom panel). c Representative images of the generated primary tumors (top panel). Data in the bottom panel represents the tumor volume generated from stable CT26^p68 cells relative to the CT26^EV cells. d Representative images depicting IHC staining of p68, RelA, Bcl-2, Bcl-xL Survivin and PCNA conducted in mice tumor-derived tissue sections. Images were captured at 200X magnification. e Comparison of the average H-scores of the indicated proteins. Error bars in (c [bottom panel] & e) represent mean (+) s.d from independent biological repeats (n = 6). P < 0.0001 is represented as **** (Student’s t-test)