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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Long noncoding RNA NEAT1 drives aggressive endometrial cancer progression via miR-361-regulated networks involving STAT3 and tumor microenvironment-related genes

Fig. 1

NEAT1 expression is upregulated in invasive, sphere-forming and TX-resistant HEC-50 cells. (a) The sphere-forming efficiency of HEC-50 cells was measured in three serial passages. The numbers of primary, secondary (generated from dissociated primary spheres) and tertiary (generated from dissociated secondary spheres) spheres are shown. (b) qRT-PCR was used to analyze CSC-related gene expression in primary and tertiary spheres. (c) Paclitaxel (TX)-resistant cell lines derived from HEC-50 cells were created as described in the Materials and Methods section. Chemosensitivity data (expressed as the IC50 and fold resistance) for the invasive, sphere-forming and TX-resistant HEC-50 derivatives and parental cells to TX treatment are summarized. (d) A heatmap of lncRNA expression shows lncRNAs with upregulated expression (NEAT1, H19, PVT1, UCA1, MIR7-3HG, SNHG16, HULC, RMST, BCAR4 and LINC00152) or downregulated expression (MEG3, GAS5, DIO3OS, MIR155HG, LINC00261, FENDRR, MIAT, TMEM161B-AS1, HAND2-AS1 and NBR2) in the invasive, sphere-forming and TX-resistant HEC-50 cells compared with that in the parental cells. (e) The NCBI Gene Expression Omnibus (GEO) dataset GSE17025 was used for profiling NEAT1 expression in stage I EC samples (n = 91) and normal endometrium samples (n = 12). (f) NEAT1 expression was higher in tissue samples from different types of cancer than in corresponding normal tissue samples from the TCGA database. (g and h) Kaplan-Meier overall survival analysis was used to assess EC patients with high or low NEAT1 expression based on the TCGA data with the UALCAN web tool (g) and KM Plotter (h). *P < 0.05

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