Fig. 6

MEF2D, ROCK1, WNT7A and KPNA4 mediate the effects of miR-361 on cell invasion and sphere formation in HEC-50 cells. (a) Western blot analysis of the indicated proteins in HEC-50 cells cotransfected with a control or miR-361 inhibitor with or without the indicated siRNA (si). (b) Cell invasion (upper) and sphere formation abilities (bottom) of HEC-50 cells cotransfected with a control or miR-361 inhibitor with or without the indicated siRNA. (c) Representative images from invasion and sphere formation assays using HEC-50 cells cotransfected with a control or miR-361 inhibitor with or without an MEF2D-specific siRNA. *P < 0.05