Fig. 6

HMGA1 and FOXM1 cooperative action in breast cancer cells governs angiogenic processes of endothelial cells. a Functional enrichment for Cellular Compartment ontology. Color intensity corresponds to the -log2(p-value) of the enrichment significance. b A Gene ontology analysis was carried out on DEGs upon HMGA1 silencing in MDA-MB-231 cells to cluster these genes in functions related to angiogenesis. The functions are ranked by p-value and the number of genes found to be regulated by HMGA1/function was annotated. c Representative immunofluorescence images of capillary-like structures (green) formed on Matrigel by HUVEC cells treated with the supernatant of MDA-MB-231 cells, which have been previously silenced for HMGA1, FOXM1 or HMGA1 and FOXM1 in combination. Serum-free medium and VEGFA were used as negative and positive controls respectively. d The number of vessels formed by HUVEC cells were counted and plotted on the graph: the white and the green bars correspond to the negative and positive controls respectively, while the supernatants from control MDA-MB-231 cells or from MDA-MB-231 cells silenced for HMGA1, FOXM1 or co-silenced for HMGA1 and FOXM1 are represented with the black, grey, light blue and purple bars respectively. The data are presented as the mean ± SD (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001; two-tailed Student’s t-test is calculated with respect to siCTRL