Fig. 5

Pharmacological inhibition of ABCC3 dysregulates STAT3 and HIF1α signaling and induces apoptosis in vivo. Tumour tissues were resected for the KPC mice enrolled to MCI-715- treated or vehicle-treated group at the end of experiment. Protein expression in vivo was verified by IHC staining as described in Methods section. Data shows representative IHC staining of FFPE pancreatic tumor tissues resected from KPC mice showing differential expression of pSTAT3 Y705 (a) and HIF1α (b) between vehicle and MCI-715- treated mice samples. Scale bar: 100 μm. The quantitative analysis of IHC staining was performed with the use of ImmunoRatio software and the results are presented as mean ± SEM of 17 (Vehicle group) and 14 (MCI-715 group) different images from at least 4 different animals; unpaired Student’s t-test was performed for statistical analysis, **p < 0.01, ***p < 0.001; (c) Representative image of the TUNEL assay performed on FFPE pancreatic tissues resected from KPC mice treated with vehicle and MCI-715 (Scale bar: 100 μm). The quantitative analysis of IHC staining was performed with the use of ImmunoRatio software as mean ± SEM of 17 (Vehicle group) and 21 (MCI-715 group) images; unpaired Student’s t-test was performed for statistical analysis, **** p < 0.0001