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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Pharmacological inhibition of ABCC3 slows tumour progression in animal models of pancreatic cancer

Fig. 6

ABCC3 as an important stroma component. a Representative IHC staining of ABCC3 expression in the pancreas of KPC control (scale bar: 100 μm) and KPC (scale bar: 50 μm) mice showing ductal (left) and stromal (right) expression of ABCC3 in KPC mice; b Western blot analysis comparing ABCC3 expression in CAFs (cancer-associated fibroblasts) and BJ (normal fibroblasts) cells; c CAFs and BJ cells were treated with indicated doses of MCI-175 and number of cells was counted after 72 h. Data presents the effect of MCI-715 treatment on the viability of CAFs and BJ cells presented as mean ± SEM of 3 independent experiments. Data is expressed as fold change of cells treated with vehicle (DMSO), one-was ANOVA was used for statistical analysis, ***p < 0.001, ****p < 0.0001; d Representative IHC staining comparing vimentin expression in FFPE pancreatic tissues from KPC mice treated with vehicle or 25 mg/kg of MCI-715 (Scale bar: 100 μm, 50 μm, 20 μm). Quantitative analysis is presented as mean ± SEM of 8 (Vehicle) and 12 (MCI-715) separate images ***p < 0.001; e Western blotting analysis of vimentin and αSMA expression in snap frozen pancreatic tissues from KPC mice treated with vehicle (n = 3) or 25 mg/kg MCI-715 (n = 4), quantitative analysis was performed with the use of ImageJ software, unpaired Student’s t-test was performed for statistical analysis, **p < 0.01, ****p < 0.0001

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