Fig. 3
Primary oesophageal squamous carcinoma cell isolation, identification and co-culture. a ESCC#1 and ESCC#2 morphology under a light microscope. b Representative nude mice with subcutaneous tumours derived from 1 × 106 ESCC#1 or ESCC#2 cells. c Representative images of H&E and IHC staining for CK5/6 and P63 in mouse xenograft tumours are shown. Scale bar of H&E staining images indicates 50 μM. Scale bar of IHC staining images indicates 20 μM d Isolated and pure NK cells from volunteer peripheral blood obtained by using an NK cell purification kit to achieve approximately 95% NK cell purity with less than 1% T cells. e RPMI1640 media (NC), the supernatant of KYSE150, EC9706, ESCC#1 or ESCC#2 cells co-cultured with NK cells. The CD56 + CD3_ NK cells were stained with CD16, NKp30, NKp44, NKp46, NKG2D, CD226, NKG2A, and CD158b and analysed by flow cytometry. f Statistical analysis of NKp30+, NKG2D+, NKp44+, NKp46+, CD226+, NKG2A+, and CD158b + NK cell percentages