Fig. 6

STAT3 feedback assay and correlation between IL-6 and multiple clinical parameters. a Flow cytometry was performed to detect the expression levels of NKP30, NKG2D, KI67 and granzyme B in co-culture models stimulated with 10 mM Stattic for 24 h in vitro. b Statistical analysis of NKp30+, NKG2D+, KI67+, and granzyme B+ NK cell percentages in three groups. Each experiment was repeated three times. c The expression level of IL-6 in paired tumour and adjacent tissues was detected by IHC. Scale bar of IHC staining images indicates 20 μM. d The percentage of IL-6-expressing cells was analysed for putative correlations with multiple clinical parameters. For the cumulative survival curves, patients were separated into two groups by the median value of tumour-infiltrating IL-6-positive cell percentages, and Kaplan-Meier plots were used to calculate cumulative survival differences. *p < 0.05, **p < 0.01, and ****p < 0.0001. Each dot represents 1 patient