Fig. 3

Nek2 induced sorafenib resistance in HCC cell lines. a, b. SMMC-7721 and MHCC-97H cells transfected with Nek2 plasmids or sh-Nek2 were treated with sorafenib for 24 h and analyzed with western blotting assay for pro-apoptotic and anti-apoptotic proteins. c. CCK-8 assays were performed to detect the growth inhibition of sorafenib on SMMC-7721 (upper panel) and MHCC-97H (bottom panel) with lentivirus Nek2 or sh-Nek2. d, e. (Left panels) SMMC-7721 and MHCC-97H cells were treated with sorafenib with lentivirus overexpression or knockdown of Nek2 and cells were analyzed by flow cytometry. (Right panels) columns, representing the total percentage of Q2 and Q4, were the average of three independent experiments. Data were presented as mean ± SEM, ns, no significance; *P<0.05; **P<0.01; ***P<0.001