Fig. 4

Nek2 induced sorafenib resistance via β-catenin in HCC cell lines. a. SMMC-7721 transfected with different combinations of lentivirus Nek2 and si-β-catenin were treated with or without sorafenib and cells were analyzed using western blotting assay. b. MHCC-97H transfected with different combinations of shRNA Nek2 and adenovirus-β-catenin were treated with or without sorafenib and cells were analyzed using western blotting assay. c, d. (Left panels) Flow cytometry was used to detect the apoptotic cells in different combinations indicated. (Right panels) columns, representing the total percentage of Q2 and Q4, were the average of three independent experiments. Data were presented as mean ± SEM, ns, no significance; *P<0.05; **P<0.01; ***P<0.001. f. Dose-dependent effects of sorafenib on the viability of SMMC-7721 (left panel) and MHCC-97H (right panel) with indicated combination of Nek2 and β-catenin