Fig. 4

MiR-501-3p from M2 macrophage-derived exosomes promotes migration and invasion of PDAC cells. a and b expression of miR-501-3p in PANC-1 and BxPC-3 cells treated with miR-501-3p mimic and Mp-Exo + miR-501-3p inhibitor was determined by RT-qPCR. c and d proliferation of PANC-1 and BxPC-3 cells treated with miR-501-3p mimic and Mp-Exo + miR-501-3p inhibitor was examined using CCK-8 assay. e-h Western blot analysis of cleaved caspase 3 and cleaved PARP proteins in PANC-1 and BxPC-3 cells treated with miR-501-3p mimic and Mp-Exo + miR-501-3p inhibitor. i-k migration and invasion of PANC-1 and BxPC-3 cells treated with miR-501-3p mimic and Mp-Exo + miR-501-3p inhibitor were examined by Transwell assay (Scale bar = 50 μM). l and m In vitro tube formation assay was applied to assess the HMEC-1 tube formation ability and quantitative analysis of branch points in PANC-1 and BxPC-3 cells treated with miR-501-3p mimic and Mp-Exo + miR-501-3p inhibitor (Scale bar = 100 μM). n-p Western blot analysis was applied to detect migration, invasion and angiogenesis-related proteins in response to miR-501-3p mimic and Mp-Exo + miR-501-3p inhibitor. * p < 0.05 vs. the NC mimic group. # p < 0.05 vs. the Mp-Exo + NC mimic group. The measurement data were expressed as mean ± standard deviation. One-way ANOVA was employed for comparison among multiple groups, followed by Tukey’s post-hoc test. The data at different time points were compared using repeated measures ANOVA, followed by Tukey’s post-hoc test. Cell experiments were repeated three times